Hawaii Pacific University | Shared Instrumentation Facilities

Established in 2005, the Shared Instrumentation Facility (SIF) houses the major scientific instruments at HPU and brings together an essential collection of labs, support equipment, staff, and other research resources. The SIF seeks to maintain high functioning research labs, provide uninterrupted access to and training on the instruments, and promote a safe working environment through extensive training and adherence to best practices.

The Labs

The SIF supports biomedical and other multidisciplinary research and integrates high tech instrumentation into HPU's science lab courses. The SIF includes instrumentation and laboratory space on the Hawaii Loa campus, including cell biology (AC 102A), chemistry (AC 102D), and multi-purpose (AC 214) research laboratories. The SIF recently added new labs in the Model Progress building in downtown Honolulu, representing a 45% expansion of HPU's biomedical research laboratories. The new space includes biochemistry (MP 401), instrumentation (MP 402), and cell culture (MP 403) labs. Research in the SIF includes natural products chemistry, organic syntheses, bioassays, toxicological analyses, cell biology, electrophysiology, analytical chemistry, and more.

Our Goals

The overarching goal of the SIF is to provide outstanding instrumentation and laboratory facilities to support chemical, biological, biomedical, and marine science research, and to support extraordinary educational activities for HPU students. Specifically, the SIF seeks to

Support state-of-the-art instrumentation and research labs at HPU to cultivate innovative and forward-looking research, while creating exceptional student research opportunities
Integrate the same high tech scientific instruments into our teaching labs to provide direct hands-on experience to all students in our laboratory courses
Provide training that promotes safe and effective use of the facilities
Provide our expertise to support and enrich student and faculty research

The SIF represents an excellent example of synergy between extramurally funded research and academics. We have developed our research enterprise to the benefit of our students, allowing them to use high tech instrumentation and gain valuable research experience as they progress through our academic programs.

Our Funding

The SIF is funded largely by the National Institutes of Health IDeA Network for Biomedical Research Excellence(INBRE) grant, which is intended to expand and develop Hawaii's competitive biomedical research capacity. The facility has also received support from the National Science Foundation's Major Research Instrumentation.

Our People

Consortium Principal Investigator

F. David Horgen, Ph.D. (dhorgen@hpu.edu)

Research Facilities Manager

Dr. Kosuke Ohgo. (kohgo@hpu.edu)

Contributing Faculty

Yongli Chen, Ph.D. (ychen@hpu.edu)

Gideon Berger, Ph.D. (gberger@hpu.edu)

Lei Wang, Ph.D. (lwang@hpu.edu)

RESEARCH TECHNICIAN

Ellis Akana (elakana@hpu.edu)

Research Assistants

Savana Vander Schuit

Jessie Nguyen

Kacy Kim

Olivia Honaker

AMber Aduja

Richelle Alfonso

Chloe Todd

STUDENT INSTRUMENTATION MENTORING PROGRAM

Amber Aduja (Co-Lead mentor)

Chloe Todd

Elena Polizotto

Gina Muench

Ivy Ammerman

Jessie Nguyen

Kacy Kim

Kaley Saludares

Kirah Boynton-Brown

MaxAllen Ponce

Olivia Honaker

Richelle Alfonso

Savana Vander Schuit (Co-Lead mentor)

SImone Duran-Nyers

Xiao Yi Wang-Beckvall

Hawaii Pacific University operates a wide range of scientific equipment in support of instruction and research. The various instruments are housed and maintained by the Shared Instrumentation Facility (SIF), the Instructional Science Laboratories, or by individual investigators. An important strategy in the development of instrumental capabilities at HPU has been to integrate research-grade equipment into our academic curriculum, thereby exposing all undergraduate science majors to state-of-the-art high tech instruments in their laboratory course work. Collectively, the instrumentation facilities at HPU have been supported by the National Science Foundation, the National Institutes of Health, and private funding. The table below catalogs HPU’s shared instrumentation, their location, and the person to contact for details on availability and potential training.

HPU's Research Instrumentation

MAJOR INSTRUMENTS

Instrument	Description	Contact	Location
FPLC (fast protein liquid chromatography) GE Healthcare AKTAprime Plus	Protein purification	Capaldo, Christopher	DSL 108
GC-MS (gas chromatograph-mass spectrometer) Agilent 7890A GC; 5975C MS	Autosampler; electron impact source; quadrapole analyzer; flame ionization detector	Ohgo, Kosuke	DSL 107
Infrared spectrometer PerkinElmer Spectrum Two FT-IR Spectrometer	Measures infrared absorption	Ohgo, Kosuke	DSL 303
Isocratic HPLC (high-performance liquid chromatography) Shimadzu LC-6A and D-Star LC; analytical and semipreparative capable; chart integrator, single wavelength UV detector	Purifies extracts or synthetic products	Ohgo, Kosuke	HL 214
LC-MS (liquid chromatography-mass spectrometer) Agilent 1200 LC and autosampler; Agilent 6530 quadrapole-time-of-flight ( QTOF )	Electrospray and atmospheric pressure chemical ionization, positive and negative mode; MS/MS; high-resolution MS. autosampler; fraction collector	Ohgo, Kosuke	DSL 107
NMR spectrometer 300 MHz Varian	1H, 13C, 15N, 31P detection with gradients, one- and two-dimensional experiments	Ohgo, Kosuke	DSL 107
Patch clamp electrophysiology system EPC-10 USB Patch Clamp Amplifier; 3 Axis Water Hydraulic Micromanipulator; Olympus IX51; Olympus TC4	Measures flux of ions across cell membranes	Chen, Yongli	DSL 002
Patch clamp ectrophysiology system Multiple components	Measures flux of ions across cell membranes	Ohgo, Kusuke	DSL 107
Plate reader BioTek Synergy HTX Multi-mode Microplate Reader	Fluorescence/absorbance/luminescence plate reader	Capaldo, Christopher	DSL 108
Preparative HPLC (high-performance liquid chromatography) Agilent 1100 preparative system	Flow up to 100 mL/min; manual injector, multi-wavelength UV detector, fraction collector	Ohgo, Kosuke	DSL 107
Scanning robotic plate reader Molecular Devices FlexStation III	Fluorescence/absorbance/luminescence plate reader with 8 channel fluidics	Ohgo, Kosuke	DSL 003
Solvent extractor Thermo Scientific Dionex ASE 350	Extraction of compound from solid samples	Ohgo, Kosuke	DSL 107
Solvent purification System Innovative Technology Puresolv	Production of anhydrous solvent	Mullane, Kimberly	DSL 106
Polarimeter Rudolph Research Analytical Autopol III	Optical rotation measurements	Ohgo, Kosuke	DSL 106

CELL AND MOLECULAR BIOLOGY SUPPORT

Instrument	Description	Contact	Location
3D Printer Formlabs Form 3B 3-Dimensional Biocompatible Printer		Green, John-Bruce	DSL 002
Analytical balance Hanchen BSM-220.4	Measures sample mass	Ohgo, Kusuke	DSL 003
Autoclave Yamato SM510	Sterilization of materials and waste	Ohgo, Kosuke	DSL 003
Autoclave Yamato SM510	Sterilization of materials and waste	Ohgo, Kosuke	DSL 004
Autoclave Yamato SM510	Sterilization of materials and waste	Capaldo, Christopher	DSL 108
Benchtop spectrometer Eppendorf BioPhotometer	Measures UV absorption	Capaldo, Christopher
Refrigerated centrifuge Eppendorf Centrifuge 5415 R	Centrifugation of cell culture samples	Capaldo, Christopher	DSL 108
Refigerated centrifuge Eppendorf Centrifuge 5810 R 15 amp version	Centrifugation of samples (mammalian cells or non-viable samples only)	Ohgo, Kosuke	DSL 002
Centrifuge VWR Clinical 100	Centrifugation of cell culture samples	Ohgo, Kosuke	DSL 002
Centrifuge VWR Clinical 200	Centrifugation of cell culture samples	Ohgo, Kosuke	DSL 003A
Centrifuge Beckman Coulter Microfuge 20R	Centrifugation of cell culture samples	Ohgo, Kosuke	DSL 002
Centrifuge VWR Symphony 2417R	Centrifugation of reagents and samples	Capaldo, Christopher	DSL 108
Centrifuge Thermo Scientific Sorvall ST40R	Centrifugation of samples	Capaldo, Christopher	DSL 108
CO2 incubator Thermo Scientific HeraCell Vios 160i	Controls humidity, CO2 concentrations, and temperature of cell cultures	Ohgo, Kosuke	DSL 003A
CO2 incubator Thermo Scientific HeraCell Vios 160i	Controls humidity, CO2 concentrations, and temperature of cell cultures	Capaldo, Christopher	DSL 108
Tube Rotator Fisher Scientific Multi-Purpoose Tube Rotator	Mixing of samples	Ohgo, Kosuke	DSL 003
Electrophoresis system Bio-Rad	Separation of DNA and protein	Ohgo, Kosuke	HL 102A
Electrophoseris system Invitrogen PowerEase Touch	Seperation of DNA and protein	Ohgo, Kosuke	DSL 003
Electrophoresis system Labnet Enduro VE10	Separation of DNA and protein	Capaldo, Christopher
Fluorescent Imager LICORbio Odyssey F Imaging System	Imaging gels	Capaldo, Christopher	DSL 108
Orbital shaker VWR 3500I	Mixing chemicals and reagents	Capaldo, Christopher
Orbital shaker GeneMate GyroMixer Variable XL	Mixing chemicals and reagents	Capaldo, Christopher	DSL 108
Plate washer BioTek ELx-50	96 well plate washing, aspirating and dispensing	Ohgo, Kosuke	DSL 003
Shaking incubator VWR 1575	Heating samples and reagents	Ohgo, Kosuke	DSL 004
Shaking incubator VWR 1575	Heating samples and reagents	Capaldo, Christopher	DSL 108
TEER measurement WPI EVOM Manual	Measuring Trans Epithelial Electrical Resistance (TEER) measurement	Ohgo, Kosuke	DSL 003
Thermocycler Hybaid, OMN-E Thermal cycler	Incubations and polymerase chain reaction experiments	Ohgo, Kosuke	DSL 002
Thermocycler MJ Research PTC-200	Incubations and polymerase chain reaction experiments	Ohgo, Kosuke	DSL 004
Thermocycler Applied Biosystems Veriti 96 Well Thermal Cycler	Incubations and polymerase chain reaction experiments	Capaldo, Christopher	DSL 108
Thermocycler BioRad T100 Thermal Cycler	Incubations and polymerase chain reaction experiments	Capaldo, Christopher	DSL 108
Thermocycler BioRad CFX96 Real-Time, C100 Touch Thermal Cycler	Incubations and real time polymerase chain reaction experiments	Capaldo, Christopher	DSL 108
Top loading balance Mettler Toledo PL3001-S	Measures sample mass	Ohgo, Kosuke	DSL 004
Water Bath Fisher Scientific Isotemp 205 Digital Water Bath	Temperature control of water for regulating temperatures	Ohgo, Kosuke	DSL 002
Water Bath Fisher Scientific Isotemp 205 Digital Water Bath	Temperature control of water for regulating temperatures	Ohgo, Kosuke	DSL 003A

CHEMISTRY AND BIOCHEMISTRY SUPPORT

Instrument	Description	Contact	Location
Analytical balance Sartorius CP124S	Measures sample mass	Ohgo, Kosuke	DSL 002
Analytical balance Sartorius CP124S	Measures sample mass	Kranbuhl, Sierra	HL 102B
Analytical balance Sartorius CP124S	Measures sample mass	Kranbuhl, Sierra	HL 212
Analytical balance Sartorius CP225D	Measures sample mass	Ohgo, Kosuke	DSL 107
Analytical balance Sartorius Secura 224-1S	Measures sample mass	Capaldo, Christopher	DSL 108
Benchtop spectrometer VWR UV-1600PC	Measures UV absorption	Ohgo, Kosuke	DSL 002
Benchtop spectrometer VWR UV-1600PC	Measures UV absorption	Capaldo, Christopher	DSL 108
Calorimeter Parr Oxygen Bomb Calorimeter	Measures heat evolved by chemical reactions	Ohgo, Kosuke	DSL 105
Convection oven Blue-M, MO1450SA	Oven to dry samples	Kranbuhl, Sierra	HL 102B
Convection oven Lindberg/BlueM, MO1450A-1	Oven to dry samples	Kranbuhl, Sierra	HL 212A
Freeze-dryer Heto PowerDry LL3000	Freeze dry various research sample and dry residual water in samples	Ohgo, Kosuke	DSL 107
Incubator Benchmark Scientific MyTemp Mini Digital	Oven to dry samples	Capaldo, Christopher	DSL 108
Incubator VWR BOD Low Temperature Refrigerated Shaking Incubator 1585	Incubator for bacterial cultures	Capaldo, Christopher	DSL 108
Muffle furnace Thermolyne 62700 Muffle Furnace	Furnace to isolate the subject material from the fuel and all of the products of combustion	Ohgo, Kosuke	DSL 001
Oven Thermolyne F62700 Furnace	Oven to dry samples/equipment	Vizza, Carmella	DSL 001
Oven Fisher Scientific Isotemp	Oven to dry samples	Ohgo, Kosuke	DSL 105
Refrigerated centrifuge Beckman Coulter Allegra 6R	Centrifugation of samples	Ohgo,Kosuke	DSL 003
Rotary evaporator Heidolph Laborota 4011	Evaporates solvents from solutions	Ohgo, Kosuke	DSL 10
Rotary evaporator IKA RV 10 CV S1	Evaporates solvents from solutions	Berger, Gideon	DSL 106
Top loading balance Sartorius CP2202S	Measures sample mass	Ohgo, Kosuke	DSL 106
Top loading balance Denver Instrument SI-403	Measures sample mass	Ohgo,Kosuke	DSL 001
Ultrasonic Cleaner BRANSON Ultrasonic Cleaner 2210	Clean glassware and dissolve solids in a solvent	Ohgo, Kosuke	DSL 106
UV/VIS spectrometer Perkin Elmer Lambda 20	Measure ultraviolet and visible light absorption	Kranbuhl, Sierra	HL 211A
UV/VIS spectrometer Perkin Elmer Lambda 25	Measure ultraviolet and visible light absorption	Kranbuhl, Sierra	HL 211A
Vacuum evaporation system Labconco CentriVap Cold Trap	Evaporates solvents from solutions	Ohgo, Kosuke	DSL 107
Vacuum evaporation system Savant SpeedVac Concentrator SPD111V; -40C cold trap	Evaporates solvents from solutions	Ohgo, Kosuke	DSL 107
Vacuum evaporation system Welch ChemStar Dry	Evaporates solvents from solutions	Ohgo, Kosuke	DSL 107
Vacuum evaporation system Welch Self-Cleaning Dry Vacuum System 2028	Evaporates solvents from solutions	Berger, Gideon	DSL 106
Water systems Millipore MilliQ Integral 5	Produces Type II and Type I water	Ohgo, Kosuke	DSL 003

ELECTROPHYSIOLOGY SUPPORT

Instrument	Description	Contact	Location
Pipette puller HEKA RIP 6	Glass pipettes for electrophysiology	Ohgo, Kosuke	DSL 002
Pipette puller Narishige PC10	Glass pipettes for electrophysiology	Ohgo, Kosuke	DSL 002

FREEZERS AND REFRIGERATORS

Instrument	Description	Contact	Location
Freezer Fisher Scientific Isotemp -86ºC Ultra-Low Temperature	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL 003
Freezer Fisher Scientific Isotemp -86°C Ultra-Low Temperature	Sample, cell, and reagent storage	Capaldo, Christopher	DSL 108
Freezer Portable Freezer Cryogenic Refrigerator -86°C Low Temperature Freezer Samples	Sample, cell, and reagent storage	Jacob, Jessica	DSL 001
Freezer Kenmore -20ºC	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL 107
Freezer Chest Freezer -20ºC	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL 105
Freezer Chest Freezer -20ºC	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL Basement
Freezer Chest Freezer -20ºC	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL Basement
Freezer Kenmore -20ºC	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL 106
Freezer Kenmore -20°C	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL 002
Freezer Frigidaire -20ºC Chest Freezer	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL 004
Freezer Fisher Scientific Isotemp -86°C Ultra-Low Temperature	Sample, cell, and reagent storage	Capaldo, Christopher	DSL 108
Freezer Kenmore -20°C	Sample, cell, and reagent storage	Capaldo, Christopher	DSL 108
Refrigerator Fisher Scientific Isotemp	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL 105
Refrigerator Turbo air Chromatography	Sample, call, and reagent storage	Ohgo, Kosuke	DSL 003
Refrigerator VWR International, R421FA14	Chemical storage	Kranbuhl, Sierra	HL 102B
Refrigerator VWR International, R421FA14	Chemical Storage	Ohgo, Kosuke	DSL 106
Refrigerator Howard-McCray, HC45	Sample storage	Kranbuhl, Sierra	HL 211A
Refrigerator Fisher Scientific Isotemp Chromatography	Sample, cell, and reagent storage	Capaldo, Christopher	DSL 108
Refrigerator Whirlpool	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL 004
Refrigerator Frigidaire FRT18KC3BW4	Sample, cell, and reagent storage	Ohgo, Kosuke	DSL 004
Refrigerator VWR Chromatography	Sample, cell, and reagent storage	Capaldo, Christopher	DSL 108
Refrigerator VWR Chromatography	Sample, cell, and reagent storage	Capaldo, Christopher	DSL 108

FUME HOODS AND BIOLOGICAL SAFETY CABINETS

Instrument	Description	Contact	Location
Biological safety cabinet laminar flow hood Thermo Scientific 1300 Series A2	Provide aseptic environment for manipulating cells in cultures (for mammalian cells only)	Capaldo, Christopher	DSL 108
Biological safety cabinet laminar flow hood Thermo Scientific 1300 Series A2	Provide aseptic environment for manipulating cells in cultures (for mammalian cells only)	Ohgo, Kosuke	DSL 003A
Biological safety cabinet laminar flow hood Thermo Scientific 1300 Series A2	Clean environment for preperation of PCR and seeding microbial cultures	Ohgo, Kosuke	DSL 004
Ductless fume hood Cleatech	Clean environment for preparation of PCR and seeding microbial cultures	Ohgo, Kosuke	DSL 004

MARINE BIOLOGY AND OCEANOGRAPHY SUPPORT

Instrument	Description	Contact	Location
CTD Sea-Bird Electronics, SBE-19-01	Conductivity, temperature and depth for oceanographic water analyses	Kranbuhl, Sierra	HPU, R/V KAHOLO
Current profiler Nortek USA, Aquadop 1 MHz	Measures marine current	Kranbuhl Sierra	HL 211A
R/V Kaholo Murakami Power Catamaran, 42'	Teaching and research vessel	Kranbuhl, Sierra	Kaneohe Bay, Coconut Island
Skiff, motor, trailer Livingston 12' skiff, 8 hp motor	Research craft	Kranbuhl, Sierra	Kaneohe Bay, Coconut Island
Skiff, motor, trailer Boston Whaler	Research craft	Kranbuhl, Sierra	Kaneohe Bay, Coconut Island

MICROSCOPES AND CAMERAS

Instrument	Description	Contact	Location
Camera Infinity, 2-3C	General use camera	Kranbuhl, Sierra	HL 213
Camera Nikon DS-L1	General use camera	Kranbuhl, Sierra	HL 211A
Compound microscope Nikon E200	General use camera	Ohgo, Kosuke	DSL 002
Compound microscope Olympus, CX31	General use camera	Kranbuhl, Sierra	HL 213
Inverted microscope Nikon TS100	Imaging cells in culture	Ohgo, Kosuke	DSL 003A
Microscope LW Scientific Revelation III	General use microscope	Ohgo, Kosuke	DSL 002
Microscope Zeiss Stemi 2000-C Trinocular Stereo Microscope	General use microscope	Capaldo, Christopher	DSL 108
Microscope, stereoscope Nikon SMZ-800	General use dissecting microscope	Kranbuhl, Sierra	HL 211A